The UMP/CMP kinase NMPK22 corresponds to the human gene CMPK1. This eukaryotic enzyme is a bifunctional enzyme that catalyzes the phosphorylation of both CMP and UMP with similar efficiency. dCMP can also act as acceptor. The enzyme plays an important role in de novo pyrimidine nucleotide biosynthesis.
hCMPK1, U/CMPK, UCK, cytidylate kinase, cytidine monophosphate kinase, CMP kinase, CTP:CMP phosphotransferase, deoxycytidylate kinase, deoxycytidine monophosphate kinase, dCMP kinase, uridylate kinase, uridine monophosphate kinase, pyrimidine nucleoside monophosphate kinase, uridine monophosphate-cytidine monophosphate phosphotransferase, ATP:UMP-CMP phosphotransferase
(1) ATP + (d)CMP = ADP + (d)CDP
(2) ATP + UMP = ADP + UDP
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM CMP
6 mM ATP
0.04 g/l NMPK22 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.