Adenylate kinase NMPK01
The adenylate kinase NMPK01 is of bacterial origin and thermostable up to 80°C. Adenylate kinases catalyze the phosphorylation of AMP or dAMP to ADP and dADP, respectively.
E-KI-0201
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Product Information
EC Number
2.7.4.3
CAS Number
9013-02-9
Synonyms
TK, 2'-deoxythymidine kinase, ATP:thymidine 5'-phosphotransferase
Catalyzed reaction(s)
ATP + AMP = 2 ADP
Properties
Origin
Bacterial
Molecular weight
26.4 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to 80°C
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM dATP
0.04 g/l NMPK01 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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