Next generation nucleic acid derivative synthesis based on innovative and sustainable biochemical methods, guaranteeing customized synthesis routed at competitive costs and with high regio- and stereoselectivity.

We are specialized: 

- in the production of hard to synthesize biocatalysts

- in the utilization of the biocatalysts for the production of intermediates and final products like nucleoside, nucleotides and sugar-1-phosphates

BioNukleo is your expert in enzymatic nucleoside synthesis. BioNukleo offers custom synthesis of modified nucleosides, nucleoside phosphorylase kits for their individual production, nucleoside and nucleotide kinases and a variety of sugar-1-phosphates for the direct production of modified nucleosides.  

Additionally, we are offering automated image analysis as a service. There is no more need for counting your cells manually, you can simply submit your Images and get detailed overview of your quantification, including a set of results-imagesNucleoside analogues have been in clinical use for almost 50 years and have become cornerstones of treatment for patients with cancer or viral infections. Furthermore, nucleoside analogues can be used as immunosuppressive drugs. Therefore, Azathioprine, a purine analogue, is used in organ transplantation and for treatment of autoimmune diseases. Cladribine also possesses specific activity on lymphocytes and has been evaluated in patients with autoimmune diseases such as rheumatoid arthritis and multiple sclerosis. The approval of several additional drugs over the past decade demonstrates that this family still possesses strong potential. 

Modified nucleosides are currently produced through a long and tedious chemical process that limits the production of new compounds. Consequently, new and more efficient synthesis methods are of high interest. We are presenting an enzymatic alternative to chemical methods using thermostable nucleoside phosphorylases. 

Despite the very impressive progress achieved in the development of chemical methods, the preparation of purine nucleosides remains a challenging problem. A common drawback of often used approaches is the indispensable introduction and subsequent removal of protective groups, which is associated with chromatographic purification in almost every step, a lack of selectivity of chemical reactions, and the problems associated with the poor regio- and stereoselectivity of chemical reactions. In contrast, the enzymatic synthesis of nucleosides by the transglycosylation reaction proceeds with strict stereo- and regioselectivity and requires organic solvents only for the isolation of the individual desired compound. The production process becomes sustainable and products with a purity >99% are achieved. Production time and costs are drastically reduced.

BioNukleo is an expert for the production of nucleoside phosphorylase enzyme kits. Consequently, customers will be able to design their own nucleosides and can order large batches of their favorite phosphorylase. We are also vending several kits of nucleoside kinases for the production of nucleotide analogues. Finally, our library of in-house synthesized high quality nucleoside analogues proofs the enzyme technology. However, BioNukleo offers custom synthesis of enzymes and nucleosides.