BioNukleo, a spin-off of Technische Universität Berlin, is a German biotech company developing new (chemo)enzymatic synthesis routes for nucleic acid derivatives.

BioNukleo's technology is based on the use of biocatalysts for the efficient synthesis of nucleic acid derivatives in one-pot and sustainable reactions.

Due to its strong competence in the expression of challenging proteins, BioNukleo set up enzyme libraries with wide substrate promiscuity. They allow for the production of a broad range of nucleoside and nucleotide analogs.