Enzymatic synthesis of modified nucleosides and nucleotides

Products


BioNukleo offers a wide range of thermophilic nucleoside phosphorylases, kinases,  sugar-1-phosphates, custom made nucleosides and enzyme kits for the enzymatic synthesis of modified nucleosides, and nucleotides.

Image-Analysis


Save time using BioNukleo's service for automated image analysis! Time-consuming manual analysis is no longer necessary,  let your images analyze and start the next experiment the next day!

Technology


BioNukleo established multiple protocols for the synthesis of modified nucleosides and is an expert for thermophilic nucleoside phosphorylases. Additionally, the production of sugar-1-phosphates is established.


Next generation nucleic acid derivative synthesis based on innovative and sustainable biochemical methods, guaranteeing customized synthesis routed at competitive costs and with high regio- and stereoselectivity.

We are specialized: 

- in the production of hard to synthesize biocatalysts

- in the utilization of the biocatalysts for the production of intermediates and final products like nucleoside, nucleotides and sugar-1-phosphates

 

BioNukleo is your expert in enzymatic nucleoside synthesis. BioNukleo offers custom synthesis of modified nucleosides, nucleoside phosphorylase kits for their individual production, nucleoside and nucleotide kinases and a variety of sugar-1-phosphates for the direct production of modified nucleosides.  

Additionally, we are offering automated image analysis as a service. There is no more need for counting your cells manually, you can simply submit your Images and get detailed overview of your quantification, including a set of results-imagesNucleoside analogues have been in clinical use for almost 50 years and have become cornerstones of treatment for patients with cancer or viral infections. Furthermore, nucleoside analogues can be used as immunosuppressive drugs. Therefore, Azathioprine, a purine analogue, is used in organ transplantation and for treatment of autoimmune diseases. Cladribine also possesses specific activity on lymphocytes and has been evaluated in patients with autoimmune diseases such as rheumatoid arthritis and multiple sclerosis. The approval of several additional drugs over the past decade demonstrates that this family still possesses strong potential. 

Modified nucleosides are currently produced through a long and tedious chemical process that limits the production of new compounds. Consequently, new and more efficient synthesis methods are of high interest. We are presenting an enzymatic alternative to chemical methods using thermostable nucleoside phosphorylases. 

 

Despite the very impressive progress achieved in the development of chemical methods, the preparation of purine nucleosides remains a challenging problem. A common drawback of often used approaches is the indispensable introduction and subsequent removal of protective groups, which is associated with chromatographic purification in almost every step, a lack of selectivity of chemical reactions, and the problems associated with the poor regio- and stereoselectivity of chemical reactions. In contrast, the enzymatic synthesis of nucleosides by the transglycosylation reaction proceeds with strict stereo- and regioselectivity and requires organic solvents only for the isolation of the individual desired compound. The production process becomes sustainable and products with a purity >99% are achieved. Production time and costs are drastically reduced.

BioNukleo is an expert for the production of nucleoside phosphorylase enzyme kits. Consequently, customers will be able to design their own nucleosides and can order large batches of their favorite phosphorylase. We are also vending several kits of nucleoside kinases for the production of nucleotide analogues. Finally, our library of in-house synthesized high quality nucleoside analogues proofs the enzyme technology. However, BioNukleo offers custom synthesis of enzymes and nucleosides. 

The Team

BioNukleo was founded in 2014 and is located at the chair of bioprocess engineering at Technical University of Berlin. The founders were funded by EXIST Forschungstransfer.

 

BioNukleo is based on the chemo-enzymatic production of modified nucleosides and nucleotides. Nucleosides and their analogues are well known for their antiviral, antibacterial and anticancer activities. The multistep and highly expensive chemical synthesis process hinder their wide spread as therapeutic agents, as well as limiting their use in biological trials. Chemo-enzymatic methods are being used as the new approach for synthesis, due to their relatively low cost, high selectoivity and being environmentally friendly. We invented an outstanding technique to synthesize nucleosides by a chemo-enzymatic method at a large scale and submitted a patent application recently.

 

Modified nucleosides and nucleotides are used, among others, in the following areas of application:

 

– Cell proliferation assays;

– Building blocks of oligonucleotides (FISH-probes, siRNAs, antisenseoligonucleotides);

– Antiviral, antimicrobial and anticancer drugs.

Image Analysis

The analysis of biological images is a key factor of scientific work. Scientists can save a lot of time with BioNukleo services as they don’t have to familiarize themselves with bio image analyzing programs nor do they need to quantify the images manually any longer. To facilitate scientists’ research, BioNukleo performs the quantification of all images captured during the projects, turning the images into numbers.

The demand for precise and fast image analysis has driven the development of image analysis programs and high content screening tools. However, after every successful experiment biologists are facing the problem of image quantification. Even if the effect is obvious, scientists have to proof their results with numbers. The quality of results improves exponentially with automated analysis and precision and objectivity are crucial for being published in an established journal.

 

You could set up a new experiment right next week, because you already know the results of your last experiment by automated image analysis.

You can compare parameters of structures in your images you never thought you could quantify.

You can proof objectivity. BioNukleo will examine your experiments for you.

No more counting through the weekend!

An automated analysis of your scientific images results not only in an objective and verifiable analysis but also in an enormous amount of data describing number, shape, distance, co-expression and many more parameters of the structures. You might be surprised how many image types are already quantifiable.

 

Check out our samples!