BioNukleo offers carefully assembled kits for the detection of (pyro-) phosphate by continuous UV-spectroscopic monitoring of 5-bromouridine phosphorolysis (PUB module, see also Eilert et al. 2022, DOI: 10.1021/acs.analchem.1c05356). The PUB module allows quantitative phosphate detection by observing the shift in UV spectrum when 5-bromouridine and phosphate are converted to 5-bromouracil by a pyrimidine phoshphorylase. Key properties of the PUB phosphate detection system are:
The extended version of this kit - including an inorganic pyrophosphatase (hydrolyzing pyrophosphate to orthophosphate) - enables the quantification of pyrophosphate in a fully analogous fashion.
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