UMP kinase NMPK05
The prokaryotic UMP kinase NMPK05 is specific for UMP as substrate and is used by prokaryotes in the de novo synthesis of pyrimidines, in contrast to eukaryotes, which use the dual-specificity enzyme UMP/CMP kinase (EC 2.7.4.14) for the same purpose. Secondary substrates are GMP and - to a much lesser extent - CMP. This enzyme is the subject of feedback regulation, being inhibited by UTP and activated by GTP. NMPK05 is thermostable up to 80°C.
E-KI-0205
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Product Information
EC Number
2.7.4.22
CAS Number
9036-23-1
Synonyms
UMPK, uridylate kinase, uridine monophosphate kinase, ATP:UMP phosphotransferase
Catalyzed reaction(s)
ATP + UMP = ADP + UDP
Properties
Origin
Bacterial
Molecular weight
26.6 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to 80°C
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM UMP
6 mM ATP
0.04 g/l NMPK05 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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