Guanylate kinase NMPK21
The guanylate kinase NMPK21 is a mesophilic enzyme of eukaryotic origin. It catalyzes the phosphorylation of GMP or dGMP to GDP and dGDP, respectively. ATP and dATP can act as phosphate donor.
E-KI-0221
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Product Information
EC Number
2.7.4.8
CAS Number
9026-59-9
Synonyms
UMPK, uridylate kinase, uridine monophosphate kinase, ATP:UMP phosphotransferase
Catalyzed reaction(s)
ATP + GMP = ADP + GDP
Properties
Origin
Eukaryotic
Molecular weight
22.0 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM GMP
6 mM ATP
0.04 g/l NMPK21 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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