Many enzymatic reactions require ATP as a co-substrate, and the availability of ATP can be a limiting factor in biocatalytic processes. To overcome this challenge, ATP regeneration systems have been developed, enabling sustainable and cost-effective biocatalytic synthesis. ATP regeneration systems involve coupling the desired biocatalytic reaction with an ATP-generating enzyme. These ATP-generating enzymes are carefully selected and optimized to work in tandem with the target biocatalyst, allowing for efficient and continuous ATP recycling during the biocatalytic process.
Advantages of ATP regeneration:
ATP regeneration by acetate kinase using acetylphosphate as phosphate donor.
ATP regeneration by creatine kinase using creatine phosphate as phosphate donor.
ATP regeneration by pyruvate kinase using phosphoenol-pyruvate as phosphate donor.