ATP regeneration systems
An ATP regeneration system is a coupled enzymatic reaction that constantly produces ATP from ADP and a secondary phosphate donor. ATP then serves as the phosphate donor for the main enzymatic reaction, e.g. the phosphorylation of a nucleoside. The incorporation of an ATP regeneration system offers several advantages:
There are several ATP regeneration systems that each consist of an enzyme and a phosphate donor, for example acetate kinase/acetyl phosphate (AcK/AcP), pyruvate kinase/phosphoenolpyruvate (PK/PEP) or polyphosphate kinase/polyphosphate (PPK/PolyP). Optimal reaction conditions, e.g. moderate or elevated reaction temperatures, depend on the organism the biocatalyst was obtained from.
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