TMP kinase NMPK04
The prokaryotic TMP kinase NMPK04 catalyzes the phosphorylation of (d)TMP, dGMP, AMP and, to a smaller extent, GMP. The enzyme is thermostable up to 80°C.
E-KI-0204
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Product Information
EC Number
2.7.4.9
CAS Number
9014-43-1
Synonyms
TMPK, thymidine monophosphate kinase, thymidylate kinase, deoxythymidine 5'-monophosphate kinase, dTMPK, dTMP kinase, thymidylic acid kinase, thymidylic kinase, ATP:dTMP phosphotransferase
Catalyzed reaction(s)
ATP + dTMP = ADP + dTDP
Properties
Origin
Bacterial
Molecular weight
24.2 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to 80°C
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM dTMP
6 mM ATP
0.04 g/l NMPK04 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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