Enzymes

BioNukleo produces a broad spectrum of nucleoside and nucleoside modifying enzymes. We have built an extensive enzyme library that includes both selective and promiscuous enzymes working under a wide variety of conditions (e.g. temperature or pH values). The enzymes were carefully selected to meet our customer's requirements. Our strong competence in expressing proteins with correct folding and modifications allows us to produce even super large or multi-subunit enzymes. 

Our enzyme library is constantly growing, if you have a specific request, that is not yet included in our portfolio, please contact us!

 

All enzymes are produced and sold exclusively for research purposes and in vitro use only. The recombinant enzymes are delivered in mg scale, larger quantities can be supplied on demand.

 

Do you need help choosing the right enzyme for your desired reaction? Please contact us or check out our enzyme activity screening service!

 

Nucleoside phosphorylases (NPs) are transferases catalyzing the reversible cleavage of the glycosidic bond of natural and modifed nucleosides. Based on their substrate spectrum they can be classified into pyrimidine nucleoside phosphorylases (thymidine phosphorylase, uridine phosphorylase, pyrimidine nucleoside phosphorylase) and purine nucleoside phosphorylases (purine nucleoside phosphorylase, methylthioadenosine phosphorylase [Ealick and Pugmire, 2002]).

Transglycosylation

By combining a pyrimidine and a purine nucleoside phosphorylase, the enzymatic synthesis of natural and modified nucleosides is possible in a one-pot, two enzyme transglycosylation reaction.

 

Nucleoside phosphorylases available at BioNukleo:

Pyrimidine nucleoside phosphorylases

Purine nucleoside phosphorylases

 

Nucleoside phosphorylases (NPs) are transferases catalyzing the reversible cleavage of the glycosidic bond of natural and modifed nucleosides. Based on their substrate spectrum they can be classified into pyrimidine nucleoside phosphorylases (thymidine phosphorylase, uridine phosphorylase, pyrimidine nucleoside phosphorylase) and purine nucleoside phosphorylases (purine nucleoside phosphorylase, methylthioadenosine phosphorylase [Ealick and Pugmire, 2002]).

Transglycosylation

By combining a pyrimidine and a purine nucleoside phosphorylase, the enzymatic synthesis of natural and modified nucleosides is possible in a one-pot, two enzyme transglycosylation reaction.

 

Nucleoside phosphorylases available at BioNukleo:

Pyrimidine nucleoside phosphorylases

Purine nucleoside phosphorylases

 

Nucleoside phosphorylases (NPs) are transferases catalyzing the reversible cleavage of the glycosidic bond of natural and modifed nucleosides. Based on their substrate spectrum they can be classified into pyrimidine nucleoside phosphorylases (thymidine phosphorylase, uridine phosphorylase, pyrimidine nucleoside phosphorylase) and purine nucleoside phosphorylases (purine nucleoside phosphorylase, methylthioadenosine phosphorylase [Ealick and Pugmire, 2002]).

Transglycosylation

By combining a pyrimidine and a purine nucleoside phosphorylase, the enzymatic synthesis of natural and modified nucleosides is possible in a one-pot, two enzyme transglycosylation reaction.

 

Nucleoside phosphorylases available at BioNukleo:

Pyrimidine nucleoside phosphorylases

Purine nucleoside phosphorylases