(d)CMP kinase NMPK02
The prokaryotic (d)CMP kinase NMPK02 specifically phosphorylates CMP (or dCMP), using ATP as the preferred phosphoryl donor. Unlike EC 2.7.4.14, a eukaryotic enzyme that phosphorylates UMP and CMP with similar efficiency, the prokaryotic enzyme phosphorylates UMP (and AMP) with much lower rates. The enzyme accepts dCMP nearly as well as it does CMP. NMPK02 is thermostable up to 80°C.
E-KI-0202
160,00 €
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Product Information
EC Number
2.7.4.25
CAS Number
Synonyms
CMPK, dCMPK, prokaryotic cytidylate kinase, deoxycytidylate kinase, deoxycytidine monophosphokinase, ATP:(d)CMP phosphotransferase
Catalyzed reaction(s)
ATP + (d)CMP = ADP + (d)CDP
Properties
Origin
Bacterial
Molecular weight
26.0 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to 80°C
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM CMP
6 mM ATP
0.04 g/l NMPK02 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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