TMPK, thymidine monophosphate kinase, thymidylate kinase, deoxythymidine 5'-monophosphate kinase, dTMPK, dTMP kinase, thymidylic acid kinase, thymidylic kinase, ATP:dTMP phosphotransferase
ATP + dTMP = ADP + dTDP
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to 80°C
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM dTMP
6 mM ATP
0.04 g/l NMPK04 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.