The prokaryotic (d)CMP kinase NMPK02 specifically phosphorylates CMP (or dCMP), using ATP as the preferred phosphoryl donor. Unlike EC 18.104.22.168, a eukaryotic enzyme that phosphorylates UMP and CMP with similar efficiency, the prokaryotic enzyme phosphorylates UMP (and AMP) with much lower rates. The enzyme accepts dCMP nearly as well as it does CMP. NMPK02 is thermostable up to 80°C.
CMPK, dCMPK, prokaryotic cytidylate kinase, deoxycytidylate kinase, deoxycytidine monophosphokinase, ATP:(d)CMP phosphotransferase
ATP + (d)CMP = ADP + (d)CDP
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to 80°C
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM CMP
6 mM ATP
0.04 g/l NMPK02 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.