The adenylate kinase NMPK01 is of bacterial origin and thermostable up to 80°C. Adenylate kinases catalyze the phosphorylation of AMP or dAMP to ADP and dADP, respectively.
TK, 2'-deoxythymidine kinase, ATP:thymidine 5'-phosphotransferase
ATP + AMP = 2 ADP
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to 80°C
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM AMP
6 mM dATP
0.04 g/l NMPK01 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.