Purine nucleoside phosphorylase N06

The purine nucleoside phosphorylase N06 is of bacterial origin and thermostable up to 80°C. Purine nucleoside phosphorylases catalyze the reversible phosphorolytic breakdown of the N-glycosidic bond of (deoxy)ribonucleosides, with the formation of the corresponding free purine base and pentose-1-phosphate. 

E-NP-2006

 

Bacterial origin, thermostable

140,00 €

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Product Information

EC Number

2.4.2.1

CAS Number

9030-21-1

Synonyms

PNP, PNPase, Pu-NPase, PUNP, NP, adenosine phosphorylase, inosine-guanosine phosphorylase, purine-nucleoside:phosphate ribosyltransferase

Catalyzed reaction(s)

(1) purine ribonucleoside + phosphate = purine + α-D-ribose 1-phosphate

(2) purine 2'-deoxyribonucleoside + phosphate = purine + 2-deoxy-α-D-ribose 1-phosphate


Properties

Origin

Bacterial

Molecular weight

34.8 kDa

Production

Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.

Other characteristics

Thermostable up to (at least) 80°C


Application

Formulation

Liquid, 50 % (v/v) glycerin

Storage

-20°C

Example reaction conditions

 

N/A

 

Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.


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