The uridine phosphorylase Y12 is a mesophilic enzyme originating from Escherichia coli. Uridine phosphorylases catalyze the reversible phosphorolytic breakdown of the N-glycosidic bond of uridine with the formation of uracil and ribose-1-phosphate.
E-NP-1012
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EC Number
2.4.2.3
CAS Number
9030-22-2
Synonyms
Udp, UP, UPase, Py-NPase, PYNP, pynpase, pyrimidine ribonucleoside phosphorylase, uridine:phosphate α-D-ribosyltransferase
Catalyzed reaction(s)
Uridine + phosphate = uracil + α-D-ribose 1-phosphate
Origin
Escherichia coli
Molecular weight
28.6 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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