Nucleoside deoxyribosyltransferase NdT02
NdT02 is a nucleoside deoxyribosyltransferase (type II) from Lactobacillus leichmannii. Type II nucleoside deoxyribosyltransferases catalyze the direct transfer of the 2-deoxyribosyl moiety from a 2′-deoxynucleoside donor to a nucleobase acceptor through a one-step reaction and allow the transfer between purines and/or pyrimidines (Pur↔Pur, Pur↔Pyr, Pyr↔Pyr).
E-NT-0002
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Product Information
EC Number
2.4.2.6
CAS Number
9026-86-2
Synonyms
NdT, nucleoside 2'-deoxyribosyltransferase type II, NDT, trans-deoxyribosylase, deoxyribose transferase, DRTase, purine(pyrimidine) nucleoside:purine(pyrimidine) deoxyribosyl transferase, nucleoside trans-N-deoxyribosylase, nucleoside:purine(pyrimidine) deoxy-D-ribosyltransferase
Catalyzed reaction(s)
2-deoxy-D-ribosyl-base1 + base2 = 2-deoxy-D-ribosyl-base2 + base1
Base1 and base2 represent various purines and pyrimidines.
Properties
Origin
Lactobacillus leichmannii
Molecular weight
19.3 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic, broad substrate spectrum
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
50 mM MES pH 6
1 mM deoxyadenosine
1 mM uracil
0.3 g/l NdT02 enzyme
40°C, 60 min
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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