Purine nucleoside phosphorylase N07
The purine nucleoside phosphorylase N07 is of bacterial origin and thermostable up to 80°C. Purine nucleoside phosphorylases catalyze the reversible phosphorolytic breakdown of the N-glycosidic bond of (deoxy)ribonucleosides, with the formation of the corresponding free purine base and pentose-1-phosphate.
E-NP-2007
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Product Information
EC Number
2.4.2.1
CAS Number
9030-21-1
Synonyms
PNP, PNPase, Pu-NPase, PUNP, NP, adenosine phosphorylase, inosine-guanosine phosphorylase, purine-nucleoside:phosphate ribosyltransferase
Catalyzed reaction(s)
(1) purine ribonucleoside + phosphate = purine + α-D-ribose 1-phosphate
(2) purine 2'-deoxyribonucleoside + phosphate = purine + 2-deoxy-α-D-ribose 1-phosphate
Properties
Origin
Bacterial
Molecular weight
30.6 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to (at least) 80°C
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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