The uridine phosphorylase Y08 is of archaeal origin and thermostable up to 80°C. Uridine phosphorylases catalyze the reversible phosphorolytic breakdown of the N-glycosidic bond of uridine with the formation of uracil and ribose-1-phosphate.
E-NP-1008
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EC Number
2.4.2.3
CAS Number
9030-22-2
Synonyms
UP, UPase, Py-NPase, PYNP, pynpase, pyrimidine ribonucleoside phosphorylase, uridine:phosphate α-D-ribosyltransferase
Catalyzed reaction(s)
uridine + phosphate = uracil + α-D-ribose 1-phosphate
Origin
Archaeal
Molecular weight
31.6 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to (at least) 80°C
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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