The AMP phosphorylase AM01 corresponds to the Thermococcus kodakarensis deoA gene and is thermostable up to at least 80°C. AMP phosphorylases catalyze the reversible conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). In addition to AMP, AM01 exhibits phosphorylase activity towards CMP, UMP and GMP. This enzyme is sold as active inclusion body.
E-AM-0001
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EC Number
2.4.2.57
CAS Number
Synonyms
Tk-DeoA, AMPP, AMPpase, nucleoside monophosphate phosphorylase, AMP:phosphate alpha-D-ribosyl 5'-phosphate-transferase
Catalyzed reaction(s)
(1) AMP + phosphate = adenine + α-D-ribose 1,5-bisphosphate
(2) CMP + phosphate = cytosine + α-D-ribose 1,5-bisphosphate
(3) UMP + phosphate = uracil + α-D-ribose 1,5-bisphosphate
Origin
Thermococcus kodakarensis
Molecular weight
55.0 kDa
Production
Recombinant from E. coli, purified by inclusion body purification. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to (at least) 80°C, activity at high temperature range (20 - 80°C)
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.