The polyphosphate kinase PPK04 is a bacterial PPK2 (class III) enzyme and thermostable up to 50°C. Polyphosphate kinases of the PPK2 (class III) family preferentially catalyze polyphosphate-driven phosphorylation (utilization of polyphosphate) of both nucleoside monophosphates and nucleoside diphosphates and can thus subtitute NMPKs and NDPKs or be used as ATP regeneration system in kinase reactions. Hyperphosphorylation of nucleotides beyond three phosphate groups may occur.
PPK, PPK2 class III, polyphosphoric acid kinase, ATP:polyphosphate phosphotransferase
(1) ADP + (phosphate)n = ATP + (phosphate)n-1
(2) AMP + (phosphate)n = ADP + (phosphate)n-1
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to 50°C, allows ATP regeneration
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6
10 mM MgCl2
5 mM ADP
6 mM polyphosphate
0.04 g/l PPK04 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.