Pyruvate kinase PK02
The pyruvate kinase PK02 is of bacterial origin and thermostable up to 80°C. Pyruvate kinases phosphorylate pyruvate to phosphoenolpyruvate using ATP, UTP, GTP, CTP, ITP or dATP as phosphate donor. By utilizing the reverse reaction PK02 can be used as ATP regeneration system in kinase reactions. Substrate preference: ADP, dADP, GDP, dGDP>>UDP>TDP, CDP, dCDP.
E-KI-0602
180,00 €
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- Available
- Ships within 5-15 working days
Product Information
EC Number
2.7.1.40
CAS Number
9001-59-6
Synonyms
PK, phosphoenolpyruvate kinase, phosphoenol transphosphorylase, fluorokinase, pyruvic kinase, pyruvate phosphotransferase, ATP:pyruvate 2-O-phosphotransferase
Catalyzed reaction(s)
ATP + pyruvate = ADP + phosphoenolpyruvate
Properties
Origin
Bacterial
Molecular weight
51.9 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable, allows ATP regeneration
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM phosphoenolpyruvate
0.04 g/l PK02 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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