Acetate kinase AcK01
The acetate kinase AcK01 is a thermostable enzyme of bacterial origin. It phosphorylates all natural (d)NDPs to the respective (d)NTPs using acetyl phosphate as phosphate donor and can be used as a substitute for nucleoside-diphosphate kinases and as an ATP regeneration system in kinase reactions.
E-KI-0401
220,00 €
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Product Information
EC Number
2.7.2.1
CAS Number
9027-42-3
Synonyms
AcK, acetokinase, AckA, AK, acetic kinase, ATP:acetate phosphotransferase
Catalyzed reaction(s)
ADP + acetyl phosphate = ATP + acetate
Properties
Origin
Bacterial
Molecular weight
46.1 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable, allows ATP regeneration
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM acetyl phosphate
0.04 g/l AcK01 enzyme
37°C, 2 h
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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