Polyphosphate kinase PPK04
The polyphosphate kinase PPK04 is a bacterial PPK2 (class III) enzyme and thermostable up to 50°C. Polyphosphate kinases of the PPK2 (class III) family preferentially catalyze polyphosphate-driven phosphorylation (utilization of polyphosphate) of both nucleoside monophosphates and nucleoside diphosphates and can thus subtitute NMPKs and NDPKs or be used as ATP regeneration system in kinase reactions. Hyperphosphorylation of nucleotides beyond three phosphate groups may occur.
E-KI-0704
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Product Information
EC Number
2.7.4.1
CAS Number
9026-44-2
Synonyms
PPK, PPK2 class III, polyphosphoric acid kinase, ATP:polyphosphate phosphotransferase
Catalyzed reaction(s)
(1) ADP + (phosphate)n = ATP + (phosphate)n-1
(2) AMP + (phosphate)n = ADP + (phosphate)n-1
Properties
Origin
Bacterial
Molecular weight
31.7 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to 50°C, allows ATP regeneration
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6
10 mM MgCl2
5 mM ADP
6 mM polyphosphate
0.04 g/l PPK04 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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