The polyphosphate kinase PPK03 is a bacterial PPK2 (class I) enzyme. Polyphosphate kinases of the PPK2 (class I) family preferentially catalyze polyphosphate-driven phosphorylation (utilization of polyphosphate) of nucleoside diphosphates and can thus subtitute nucleoside-diphosphate kinases or be used as ATP regeneration system in kinase reactions.
E-KI-0703
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EC Number
2.7.4.1
CAS Number
9026-44-2
Synonyms
PPK, PPK2 class I, polyphosphoric acid kinase, ATP:polyphosphate phosphotransferase
Catalyzed reaction(s)
ADP + (phosphate)n = ATP + (phosphate)n-1
Origin
Bacterial
Molecular weight
32.4 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to 45°C, allows ATP regeneration
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6
10 mM MgCl2
5 mM ADP
6 mM polyphosphate
0.04 g/l PPK03 enzyme
45°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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