The polyphosphate kinase PPK03 is a bacterial PPK2 (class I) enzyme. Polyphosphate kinases of the PPK2 (class I) family preferentially catalyze polyphosphate-driven phosphorylation (utilization of polyphosphate) of nucleoside diphosphates and can thus subtitute nucleoside-diphosphate kinases or be used as ATP regeneration system in kinase reactions.
PPK, PPK2 class I, polyphosphoric acid kinase, ATP:polyphosphate phosphotransferase
ADP + (phosphate)n = ATP + (phosphate)n-1
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to 45°C, allows ATP regeneration
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6
10 mM MgCl2
5 mM ADP
6 mM polyphosphate
0.04 g/l PPK03 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.