The polyphosphate kinase PPK02 is a bacterial PPK1 and thermostable up to 70°C. Polyphosphate kinases of the PPK1 family catalyze the formation of polyphosphate from ATP. By utilizing the reverse reaction PPK02 can be used as ATP regeneration system in kinase reactions.
PPK, PPK1, polyphosphoric acid kinase, ATP:polyphosphate phosphotransferase
ATP + (phosphate)n = ADP + (phosphate)n+1
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to 70°C, allows ATP regeneration
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6
10 mM MgCl2
5 mM ADP
6 mM polyphosphate
0.04 g/l PPK02 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.