Polyphosphate kinase PPK02
The polyphosphate kinase PPK02 is a bacterial PPK1 and thermostable up to 70°C. Polyphosphate kinases of the PPK1 family catalyze the formation of polyphosphate from ATP. By utilizing the reverse reaction PPK02 can be used as ATP regeneration system in kinase reactions.
E-KI-0702
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Product Information
EC Number
2.7.4.1
CAS Number
9026-44-2
Synonyms
PPK, PPK1, polyphosphoric acid kinase, ATP:polyphosphate phosphotransferase
Catalyzed reaction(s)
ATP + (phosphate)n = ADP + (phosphate)n+1
Properties
Origin
Bacterial
Molecular weight
86.4 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to 70°C, allows ATP regeneration
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6
10 mM MgCl2
5 mM ADP
6 mM polyphosphate
0.04 g/l PPK02 enzyme
60°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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