The creatine kinase (M-type) CK01 corresponds to the human CKM gene. The enzyme reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). CK01 can be used as ATP regeneration system in kinase reactions.
hCKM, CK, creatine kinase muscle, M-CK, creatine kinase M chain, creatine phosphokinase M-type, CPK-M, ATP:creatine phosphotransferase
ATP + creatine = ADP + H+ + N-phosphocreatine
69.3 kDa (including GST-Tag)
Recombinant from E. coli, purified by GST-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Mesophilic, allows ATP regeneration
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM creatine phosphate
0.04 g/l CK01 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.