Creatine kinase (M-type) CK01
The creatine kinase (M-type) CK01 corresponds to the human CKM gene. The enzyme reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). CK01 can be used as ATP regeneration system in kinase reactions.
E-KI-0501
220,00 €
incl. VAT, shipping costs apply
- Available
- Ships within 5-15 working days
Product Information
EC Number
2.7.3.2
CAS Number
9001-15-4
Synonyms
hCKM, CK, creatine kinase muscle, M-CK, creatine kinase M chain, creatine phosphokinase M-type, CPK-M, ATP:creatine phosphotransferase
Catalyzed reaction(s)
ATP + creatine = ADP + H+ + N-phosphocreatine
Properties
Origin
Homo sapiens
Molecular weight
69.3 kDa (including GST-Tag)
Production
Recombinant from E. coli, purified by GST-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic, allows ATP regeneration
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM creatine phosphate
0.04 g/l CK01 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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