The acetate kinase AcK01 is a thermostable enzyme of bacterial origin. It phosphorylates all natural (d)NDPs to the respective (d)NTPs using acetyl phosphate as phosphate donor and can be used as a substitute for nucleoside-diphosphate kinases and as an ATP regeneration system in kinase reactions.
AcK, acetokinase, AckA, AK, acetic kinase, ATP:acetate phosphotransferase
ADP + acetyl phosphate = ATP + acetate
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable, allows ATP regeneration
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM ADP
6 mM acetyl phosphate
0.04 g/l AcK01 enzyme
37°C, 2 h
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.