Pyrimidine nucleoside phosphorylase Y06

The pyrimidine nucleoside phosphorylase Y06 is a mesophilic enzyme of bacterial origin. Pyrimidine nucleoside phosphorylases catalyze the reversible phosphorolytic breakdown of the N-glycosidic bond of the pyrimidine nucleosides uridine, thymidine and 2'-deoxyuridine with the formation of the corresponding pyrimidine base and pentose-1-phosphate. The enzyme does not distinguish between alpha-D-ribose 1-phosphate and 2-deoxy-alpha-D-ribose 1-phosphate in the synthetic direction.

E-NP-1006

 

Bacterial origin, mesophilic

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Product Information

EC Number

2.4.2.2

CAS Number

9055-35-0

Synonyms

PyNP, Py-NPase, PYNP, pynpase, pyrimidine ribonucleoside phosphorylase, pyrimidine-nucleoside:phosphate (2'-deoxy)-α-D-ribosyltransferase

Catalyzed reaction(s)

(1) uridine + phosphate = uracil + α-D-ribose 1-phosphate

(2) 2'-deoxyuridine + phosphate = uracil + 2-deoxy-α-D-ribose 1-phosphate

(3) thymidine + phosphate = thymine + 2-deoxy-α-D-ribose 1-phosphate


Properties

Origin

Bacterial

Molecular weight

27.3 kDa

Production

Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.

Other characteristics

Mesophilic


Application

Formulation

Liquid, 50 % (v/v) glycerin

Storage

-20°C

Example reaction conditions

N/A

 

Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.


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