The uridine/cytidine kinase NK15 originates from the humanUCK2 gene (isoform 1). The enzyme phosphorylates both uridine and cytidine (and their analogs) to their respective monophosphate forms. It does not phosphorylate deoxyribonucleosides or purine ribonucleosides. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.
hUCK2, UCK, URK1, pyrimidine ribonucleoside kinase, uridine-cytidine kinase, uridine kinase, uridine phosphokinase, ATP:uridine 5'-phosphotransferase, ATP:uridine/cytidine 5'-phosphotransferase
(1) ATP + uridine = ADP + UMP
(2) ATP + cytidine = ADP + CMP
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Broad acceptance of uridine- and cytidine-related ribonucleosides
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM uridine
6 mM ATP
0.04 g/l NK15 enzyme
37°C, 1 h
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.