Adenosine kinase NK14
The adenosine kinase NK14 originates from the human ADK gene. It catalyzes the phosphorylation of the purine nucleoside adenosine to AMP and also accepts deoxyadenosine and other related nucleoside analogs as substrates. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.
E-KI-0114
180,00 €
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Product Information
EC Number
2.7.1.20
CAS Number
9027-72-9
Synonyms
hADK, AK, ATP:adenosine 5'-phosphotransferase
Catalyzed reaction(s)
ATP + adenosine = ADP + AMP
Properties
Origin
Homo sapiens
Molecular weight
40.1 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic
Specific for adenosine-related substrates
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM adenosine
6 mM ATP
0.04 g/l NK14 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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