Deoxycytidine kinase NK13
The deoxycytidine kinase NK13 originates from the human DCK gene. It predominantly converts deoxycytidine to dCMP, but also accepts deoxyguanosine and deoxyadenosine as substrates. The enzyme has broad substrate specificity and does not display selectivity based on the chirality of the substrate. It is an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. All natural nucleoside triphosphates (except dCTP) can act as donors. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.
E-KI-0113
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Product Information
EC Number
2.7.1.74
CAS Number
9039-45-6
Synonyms
hDCK, dCK, 2'-deoxycytidine kinase, Ara-C kinase, arabinofuranosylcytosine kinase, NTP:deoxycytidine 5'-phosphotransferase
Catalyzed reaction(s)
NTP + deoxycytidine = NDP + dCMP
Properties
Origin
Homo sapiens
Molecular weight
31.9 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic
Broad substrate spectrum
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM deoxycytidine
6 mM ATP
0.04 g/l NK13 enzyme
37°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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