Deoxynucleoside kinase NK12
The deoxynucleoside kinase NK12 is a mesophilic enzyme originating from Drosophila melanogaster. It phosphorylates deoxynucleosides to dNMPs using (d)ATP as phosphate donor. The enzyme differs from other 2'-deoxyribonucleoside kinases [EC 2.7.1.76 (2'-deoxyadenosine kinase) and EC 2.7.1.113 (deoxyguanosine kinase)] in its broad specificity for all four common deoxynucleosides. The quasi-irreversible transfer of phosphate from the high energy donor to the nucleoside enables high NMP yields.
E-KI-0112
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Product Information
EC Number
2.7.1.145
CAS Number
52227-81-3
Synonyms
Dm-dNK, dNK, multispecific deoxynucleoside kinase, multisubstrate deoxyribonucleoside kinase, multifunctional deoxynucleoside kinase, ATP:deoxyribonucleoside 5'-phosphotransferase
Catalyzed reaction(s)
ATP + 2'-deoxyribonucleoside = ADP + 2'-deoxyribonucleoside 5'-phosphate
Properties
Origin
Drosophila melanogaster
Molecular weight
30.5 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic
Broad substrate spectrum
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM deoxycytidine
6 mM ATP
0.04 g/l NK12 enzyme
37°C, 30 min
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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