Thymidine kinase NK02
Thermostable enzyme of bacterial origin. Phosphorylates thymidine and its derivatives to the respective NMPs using ATP as phosphate donor. Deoxycytidine, deoxyguanosine, deoxyinosine, uridine and deoxyuridine can also act as acceptor, dGTP can act as a donor. The quasi-irreversible transfer of phosphate from the high energy donor to the nucleoside enables high NMP yields.
E-KI-0102
160,00 €
incl. VAT, shipping costs apply
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- Ships within 5-15 working days
Product Information
EC Number
2.7.1.21
CAS Number
9002-06-6
Synonyms
TK, 2'-deoxythymidine kinase, ATP:thymidine 5'-phosphotransferase
Catalyzed reaction(s)
ATP + thymidine = ADP + dTMP
Properties
Origin
Bacterial
Molecular weight
22.1 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to 50°C
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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