Deoxycytidine kinase NK13

The deoxycytidine kinase NK13 originates from the human DCK gene. It predominantly converts deoxycytidine to dCMP, but also accepts deoxyguanosine and deoxyadenosine as substrates. The enzyme has broad substrate specificity and does not display selectivity based on the chirality of the substrate. It is an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. All natural nucleoside triphosphates (except dCTP) can act as donors. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.



Human DCK, mesophilic

180,00 €

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Product Information

EC Number

CAS Number



hDCK, dCK, 2'-deoxycytidine kinase, Ara-C kinase, arabinofuranosylcytosine kinase, NTP:deoxycytidine 5'-phosphotransferase

Catalyzed reaction(s)

NTP + deoxycytidine = NDP + dCMP



Homo sapiens

Molecular weight

31.9 kDa


Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.

Other characteristics


Broad substrate spectrum



Liquid, 50 % (v/v) glycerin



Example reaction conditions

70 mM Tris-HCl pH 7.6 (at 37°C)

10 mM MgCl2

5 mM deoxycytidine

6 mM ATP

0.04 g/l NK13 enzyme

37°C, overnight 


Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.

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