The deoxycytidine kinase NK13 originates from the human DCK gene. It predominantly converts deoxycytidine to dCMP, but also accepts deoxyguanosine and deoxyadenosine as substrates. The enzyme has broad substrate specificity and does not display selectivity based on the chirality of the substrate. It is an essential enzyme for the phosphorylation of numerous nucleoside analogs widely employed as antiviral and chemotherapeutic agents. All natural nucleoside triphosphates (except dCTP) can act as donors. The quasi-irreversible transfer of phosphate from the high energy phosphate donor to the nucleoside enables high NMP yields.
hDCK, dCK, 2'-deoxycytidine kinase, Ara-C kinase, arabinofuranosylcytosine kinase, NTP:deoxycytidine 5'-phosphotransferase
NTP + deoxycytidine = NDP + dCMP
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Broad substrate spectrum
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM deoxycytidine
6 mM ATP
0.04 g/l NK13 enzyme
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.