The ADP-specific glucose/glucosamine kinase GK01 is of archaeal origin and thermostable up to (at least) 80°C. This enzyme catalyzes the phosphorylation of glucose or glucosamine to glucose 6-phosphate and glucosamine 6-phosphate, respectively. It is highly specific for ADP as phosphate donor. No activity can be detected when ADP is replaced by ATP, GDP, phosphoenolpyruvate, diphosphate or polyphosphate.
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GK, ADP-specific glucokinase, ADP-dependent glucokinase, ADP:D-glucose/D-glucosamine 6-phosphotransferase
(1) ADP + D-glucose = AMP + D-glucose 6-phosphate
(2) ADP + D-glucosamine = AMP + D-glucosamine 6-phosphate
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Thermostable up to (at least) 80°C
Liquid, 50 % (v/v) glycerin
Example reaction conditions
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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