Ribonucleoside hydrolase H03
The ribonucleoside hydrolase H03 corresponds to the Escherichia coli rihC gene. Ribonucleoside hydrolases catalyze the hydrolysis of nucleosides to ribose and nucleobase. H03 hydrolyzes both purine and pyrimidine ribonucleosides with a broad substrate specificity. Activity decreases in the order uridine > xanthosine > inosine > adenosine > cytidine > guanosine. The enzyme is strictly specific for ribonucleosides.
E-NH-0003
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Product Information
EC Number
3.2.2.1
3.2.2.8
CAS Number
9025-44-9
37288-60-1
Synonyms
Ec-RihC, RNH, Ec-YaaF, non-specific ribonucleoside hydrolase, purine-nucleoside ribohydrolase
Catalyzed reaction(s)
(1) purine nucleoside + H2O = D-ribose + purine base
(2) pyrimidine nucleoside + H2O = D-ribose + pyrimidine base
Properties
Origin
Escherichia coli
Molecular weight
34.0 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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