The ADP-specific glucose/glucosamine kinase GK01 is of archaeal origin and thermostable up to (at least) 80°C. This enzyme catalyzes the phosphorylation of glucose or glucosamine to glucose 6-phosphate and glucosamine 6-phosphate, respectively. It is highly specific for ADP as phosphate donor. No activity can be detected when ADP is replaced by ATP, GDP, phosphoenolpyruvate, diphosphate or polyphosphate.
E-KI-0801
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EC Number
2.7.1.147
CAS Number
173585-07-4
Synonyms
GK, ADP-specific glucokinase, ADP-dependent glucokinase, ADP:D-glucose/D-glucosamine 6-phosphotransferase
Catalyzed reaction(s)
(1) ADP + D-glucose = AMP + D-glucose 6-phosphate
(2) ADP + D-glucosamine = AMP + D-glucosamine 6-phosphate
Origin
Archaeal
Molecular weight
52.5 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to (at least) 80°C
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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