The terminal deoxynucleoside transferase TdT01 is a mesophilic enzyme of eukaryotic origin. This enzyme is a template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator one nucleotide at a time. It cannot initiate a chain de novo. The nucleoside may be ribo- or 2'-deoxyribo-.
TdT, Dntt, DNA nucleotidylexotransferase, terminal deoxyribonucleotidyltransferase, terminal addition enzyme, deoxynucleotidyl terminal transferase, deoxyribonucleic acid nucleotidyltransferase, deoxyribonucleic nucleotidyltransferase, terminal deoxynucleotide transferase, 2'-deoxyribonucleoside-5'-triphosphate:DNA deoxynucleotidylexotransferase
2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Liquid, 50 % (v/v) glycerin
Example reaction conditions
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.