Poly(U) polymerase PUP01
The poly(U) polymerase PUP01 is a mesophilic enzyme of eukaryotic origin. The enzyme catalyzes the terminal uridylation of mRNAs with short poly(U) tails, hence facilitating global mRNA decay. PUP requires an oligoribonucleotide or polyribonucleotide with a free terminal 3'-OH as a primer. Also possesses a weak poly(A) polymerase (PAP) activity.
E-PP-0001
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Product Information
EC Number
2.7.7.52
CAS Number
78519-53-6
Synonyms
PUP, Cid1, RNA uridylyltransferase, terminal uridylyltransferase, TUT, TUTase, non-canonical poly(A) polymerase, UTP:RNA uridylyltransferase
Catalyzed reaction(s)
(1) UTP + RNAn = diphosphate + RNAn+1
(2) ATP + RNAn = diphosphate + RNAn+1
Properties
Origin
Eukaryotic
Molecular weight
47.7 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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