Polynucleotide kinase PNK01
The polynucleotide kinase PNK01 is a modified version of the Escherichia virus T4 PseT enzyme. This enzyme catalyzes the phosphorylation of 5'-dephosphorylated DNA and also acts on 5'-dephosho-RNA 3'-mononucleotides. Three point mutations result in complete loss of 3' phosphatase activity.
E-KI-1001
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Product Information
EC Number
2.7.1.78
CAS Number
37211-65-7
Synonyms
PseT modified, PNK, ATP:5'-dephosphopolynucleotide 5'-phosphatase, PNK, polynucleotide 5'-hydroxyl kinase, 5'-hydroxyl polyribonucleotide kinase, 5'-hydroxyl RNA kinase, DNA 5'-hydroxyl kinase, DNA kinase, polynucleotide 5'-hydroxy-kinase, ATP:5'-dephosphopolynucleotide 5'-phosphotransferase
Catalyzed reaction(s)
ATP + 5'-dephospho-DNA = ADP + 5'-phospho-DNA
Properties
Origin
Escherichia virus T4
Molecular weight
36.0 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic, lacks 3'-phosphatase activity
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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