Ribonucleoside hydrolase H02
The ribonucleoside hydrolase H02 corresponds to the Escherichia coli rihB gene. Ribonucleoside hydrolases catalyze the irreversible hydrolysis of nucleosides to ribose and nucleobase. H02 is stricktly specific for ribonucleosides and accepts the pyrimidine nucleosides cytidine and uridine with clear preference for cytidine over uridine. Purine ribonucleosides are also accepted, but conversion is much slower.
E-NH-0002
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Product Information
EC Number
3.2.2.8
CAS Number
37288-60-1
Synonyms
Ec-RihB, RNH, Ec-YeiK, ribosylpyrimidine nucleosidase, pyrimidine nucleoside hydrolase, N-ribohydrolase, N-ribosylpyrimidine nucleosidase, pyrimidine-nucleoside ribohydrolase
Catalyzed reaction(s)
pyrimidine nucleoside + H2O = D-ribose + pyrimidine base
Properties
Origin
Escherichia coli
Molecular weight
35.1 kDa
Production
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Mesophilic
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
N/A
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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