The nucleoside-diphosphate kinase NDPK32 corresponds to the human gene NME2. It catalyzes the phosphorylation of nucleoside diphosphates to nucleoside triphosphates.
hNME2, NDPK, nucleoside 5'-diphosphate kinase, nucleoside diphosphokinase, nucleotide phosphate kinase, NDP kinase, ATP:nucleoside-diphosphate phosphotransferase
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Recombinant from E. coli, purified by His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Mesophilic, broad substrate spectrum
Liquid, 50 % (v/v) glycerin
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM GDP
6 mM ATP
0.04 g/l NDPK33 enzyme
37°C, [reaction time]
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.