Nucleoside-diphosphate kinase NDPK32
The nucleoside-diphosphate kinase NDPK32 is of bacterial origin and thermostable up to 50°C. It catalyzes the phosphorylation of nucleoside diphosphates to nucleoside triphosphates.
E-KI-0332
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Product Information
EC Number
2.7.4.6
CAS Number
9026-51-1
Synonyms
NDPK, nucleoside 5'-diphosphate kinase, nucleoside diphosphokinase, nucleotide phosphate kinase, NDP kinase, ATP:nucleoside-diphosphate phosphotransferase
Catalyzed reaction(s)
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Properties
Origin
Bacterial
Molecular weight
16.6 kDa
Production
Recombinant from E. coli, purified by heat treatment and His6-Tag affinity chromatography. Produced and sold for research purposes and in vitro use only.
Other characteristics
Thermostable up to 50°C, broad substrate spectrum
Application
Formulation
Liquid, 50 % (v/v) glycerin
Storage
-20°C
Example reaction conditions
70 mM Tris-HCl pH 7.6 (at 37°C)
10 mM MgCl2
5 mM GDP
6 mM ATP
0.04 g/l NDPK32 enzyme
50°C, overnight
Please note that optimal reaction conditions - especially reaction time and/or enzyme concentration - may vary depending on the substrate.
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