E. coli uridine phosphorylase

 

Amount: 400 µg

Concentration: 0.4 mg/ml

Y12

120,00 €

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Catalog Number

Y12



EC Number

2.4.2.3



CAS Number

9030-22-2



Description

Catalyzes the reversible phosphorylytic cleavage of uridine and deoxyuridine to uracil and ribose- or deoxyribose-1-phosphate. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis. 



Catalyzed reactions

PyNP reaction
Reversible reaction: phosphorolysis of pyrimidine nucleosides or ribosylation of pyrimidines using PyNP
Transglycosylation
Transglycosylation reaction using PNP and PyNP


Origin

Escherichia coli



Production

Recombinant from E. coli, purified with His6-tag



Molecular weight

≈ 29 kDa



Optimal reaction conditions

37°C, pH 7.0 - 8.0



Preferred natural substrate

Uridine



Example substrates

5-bromo-2'-deoxyuridine, 5-bromouridine, 5-fluoro-2'-deoxyuridine, 5-methyluridine



Formulation

Liquid, formulation with glycerin



Storage

4°C



Synonyms

apUP, L-UrdPase, pynpase, pyrimidine nucleoside phosphorylase, pyrimidine phosphorylase, StUPh, udp, UDRPase uridine:phosphate alpha-D-ribosyltransferase



Comments

Produced and sold for research purposes and in vitro use only

Structure of UP from Escherichia coli (DOI: 10.2210/pdb1RXU/pdb)



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