E. coli thymidine phosphorylase

 

Amount: 400 µg

Concentration: 0.4 mg/ml

Y11

120,00 €

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Catalog Number

Y11



EC Number

2.4.2.4



CAS Number

9030-23-3



Description

The enzymes which catalyze the reversible phosphorolysis of pyrimidine nucleosides are involved in the degradation of these compounds and in their utilization as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis.



Catalyzed reactions

PyNP reaction
Reversible reaction: phosphorolysis of pyrimidine nucleosides or ribosylation of pyrimidines using PyNP
Transglycosylation
Transglycosylation reaction using PNP and PyNP


Origin

Escherichia coli



Production

Recombinant from E. coli, purified with His6-tag



Molecular weight

≈ 49 kDa



Optimal reaction conditions

37°C, pH 7.0 - 8.0



Preferred natural substrate

Thymidine



Example substrates

3'-amino-3'deoxythymidine, 3'-deoxy-2',3'-didehydrothymidine, 4-thiothymidine, 5'-amino-5'-deoxythymidine, 5'-chloro-5'deoxythymidine, 5'-deoxythymidine, 5-nitro-2'-deoxyuridine



Formulation

Liquid, formulation with glycerin



Storage

4°C



Synonyms

deoA, TdRPase, Tpase, thymidine-orthophosphate deoxyribosyltransferase, deoxythymidine phosphorylase, dthdpase, pyrimidine phosphorylase, platelet-derived endothelial cell growth, gliostatin



Comments

Produced and sold for research purposes and in vitro use only

Example structure of PyNP from Geobacillus stearothermophilus (DOI: 10.2210/pdb1BRW/pdb)



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