Prokaryotic pyrimidine nucleoside phosphorylase 1
Amount: 400 µg
Concentration: 0.4 mg/ml
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Catalog Number
Y01
EC Number
2.4.2.2
CAS Number
9055-35-0
Description
Catalyzes phosphorolysis of the pyrimidine nucleosides uridine, thymidine and 2'-deoxyuridine with the formation of the corresponding pyrimidine base and ribose-1-phosphate. The reaction is reversible, and the enzyme does not distinguish between alpha-D-ribose 1-phosphate and 2-deoxy-alpha-D-ribose 1-phosphate in the synthetic direction.
Catalyzed reactions
Origin
Bacterial
Production
Recombinant from E. coli, purified with His6-tag
Molecular weight
≈ 47 kDa
Optimal reaction conditions
50°C, pH 7.0 - 9.0
Preferred natural substrate
Thymidine
Example substrates
Pyrimidines with 2'-amino- or 2'-deoxysugars or with ethynyl-substituents
Other characteristics
Activity at high temperature range (20 - 80°C)
Formulation
Liquid, formulation with glycerin
Storage
4°C
Synonyms
Py-NPase, PYNP, pynpase, pyrimidine ribonucleoside phosphorylase
Comments
Produced and sold for research purposes and in vitro use only
Example structure of PyNP from Geobacillus stearothermophilus (DOI: 10.2210/pdb1BRW/pdb)
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