Prokaryotic pyrimidine nucleoside phosphorylase 1

 

Amount: 400 µg

Concentration: 0.4 mg/ml

Y01

120,00 €

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Catalog Number

Y01



EC Number

2.4.2.2



CAS Number

9055-35-0



Description

Catalyzes phosphorolysis of the pyrimidine nucleosides uridine, thymidine and 2'-deoxyuridine with the formation of the corresponding pyrimidine base and ribose-1-phosphate. The reaction is reversible, and the enzyme does not distinguish between alpha-D-ribose 1-phosphate and 2-deoxy-alpha-D-ribose 1-phosphate in the synthetic direction.



Catalyzed reactions

PyNP reaction
Reversible reaction: phosphorolysis of pyrimidine nucleosides or ribosylation of pyrimidines using PyNP
Transglycosylation
Transglycosylation reaction using PNP and PyNP


Origin

Bacterial



Production

Recombinant from E. coli, purified with His6-tag



Molecular weight

≈ 47 kDa



Optimal reaction conditions

50°C, pH 7.0 - 9.0



Preferred natural substrate

Thymidine



Example substrates

Pyrimidines with 2'-amino- or 2'-deoxysugars or with ethynyl-substituents



Other characteristics

Activity at high temperature range (20 - 80°C)



Formulation

Liquid, formulation with glycerin



Storage

4°C



Synonyms

Py-NPase, PYNP, pynpase, pyrimidine ribonucleoside phosphorylase



Comments

Produced and sold for research purposes and in vitro use only

Example structure of PyNP from Geobacillus stearothermophilus (DOI: 10.2210/pdb1BRW/pdb)



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