E. coli purine nucleoside phosphorylase

 

Amount: 400 µg

Concentration: 0.4 mg/ml

N11

120,00 €

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Catalog Number

N11



EC Number

2.4.2.1



CAS Number

9030-21-1



Description

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.



Catalyzed reactions

PNP reaction
Reversible reaction: phosphorolysis of purine nucleosides or ribosylation of purines using PNP
Transglycosylation
Transglycosylation reaction using PNP and PyNP


Origin

Escherichia coli



Production

Recombinant from E. coli, purified with His6-tag



Molecular weight

≈ 27 kDa



Optimal reaction conditions

37°C, pH 7.0 - 8.0



Preferred natural substrate

Inosine



Example substrates

1-methylguanosine, 2',3'-dideoxyinosine, 7-methylinosine, nicotinamide riboside



Formulation

Liquid, formulation with glycerin



Storage

4°C



Synonyms

EcPNP, DeoD, PNPase, Pu-NPase, PUNP, NP, adenosine phosphorylase, inosine-guanosine phosphorylase



Comments

Produced and sold for research purposes and in vitro use only

Structure of PNP from Escherichia coli (DOI: 10.2210/pdb1ECP/pdb)



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