Amount: 400 µg
Concentration: 0.4 mg/ml
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Catalog Number
N08
EC Number
2.4.2.1
CAS Number
9030-21-1
Description
The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.
Catalyzed reactions
Origin
Archaeal
Production
Recombinant from E. coli, purified with His6-tag
Molecular weight
≈ 27 kDa
Optimal reaction conditions
80°C, pH 7.0 - 9.0
Preferred natural substrate
Inosine
Example substrates
Purines with 2'-amino-, 2'-fluoroarabino- or 2'- and/or 3'-halogenated sugars
Other characteristics
Very high temperature stability
Formulation
Liquid, formulation with glycerin
Storage
4°C
Synonyms
PNPase, Pu-NPase, PUNP, NP, adenosine phosphorylase, inosine-guanosine phosphorylase
Comments
Produced and sold for research purposes and in vitro use only
Example structure of PNP from Thermotoga maritima (DOI: 10.2210/pdb1VMK/pdb)