Archaeal purine nucleoside phosphorylase

 

Amount: 400 µg

Concentration: 0.4 mg/ml

N08

120,00 €

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Catalog Number

N08



EC Number

2.4.2.1



CAS Number

9030-21-1



Description

The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate.



Catalyzed reactions

PNP reaction
Reversible reaction: phosphorolysis of purine nucleosides or ribosylation of purines using PNP
Transglycosylation
Transglycosylation reaction using PNP and PyNP


Origin

Archaeal



Production

Recombinant from E. coli, purified with His6-tag



Molecular weight

≈ 27 kDa



Optimal reaction conditions

80°C, pH 7.0 - 9.0



Preferred natural substrate

Inosine



Example substrates

Purines with 2'-amino-, 2'-fluoroarabino- or 2'- and/or 3'-halogenated sugars



Other characteristics

Very high temperature stability



Formulation

Liquid, formulation with glycerin



Storage

4°C



Synonyms

PNPase, Pu-NPase, PUNP, NP, adenosine phosphorylase, inosine-guanosine phosphorylase



Comments

Produced and sold for research purposes and in vitro use only

Example structure of PNP from Thermotoga maritima (DOI: 10.2210/pdb1VMK/pdb)



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